| | 1 | == Dataset upload == |
| | 2 | |
| | 3 | Some important things to keep in mind while uploading data: |
| | 4 | |
| | 5 | * Scata will reverese-complement sequences in order to make sure that the 5' primer is found in the 5' end |
| | 6 | * If you give both 5' and 3' primer, any sequence where one of the primers cannot be found will be discarded |
| | 7 | * Scata does not yet understand degenrations in the priming sites. If you have degenerated bases, please lower the |
| | 8 | matching threshold to account for this, as N, R, Y etc does not match any base. |
| | 9 | * Scata takes priming site sequences, not the primer sequence. |
| | 10 | * Make sure you understand your Quality scores, and adjust the parameters accordingly. The defaults should work |
| | 11 | ok with new 454 data. |
| | 12 | |
